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论文信息：The determination of Plutella xylostella (L.)GSTs (PxGSTs) involved in the detoxification metabolism of Tolfenpyrad. Yifan Li, Hong Sun, Zhen Tian, Xinxin Su, Yue Li, Xuan Ye, Yifei Zhou, Shengli Zheng, Jiyuan Liu* and Yalin Zhang* .Pest Management Science.2020
BACKGROUND: Insect glutathione S-transferases (GSTs) play a crucial role in insecticides detoxification. However, there remains a distinct lack of information regarding the role of GSTs in the detoxification of Tolfenpyrad (TFP) in insects.
RESULTS: Real-time quantitative PCR showed significant up-regulation of PxGSTs after exposure to TFP for 6 h. An In vitro inhibition assay showed that TFP could inhibit PxGSTδ, PxGSTε and PxGSTσ, and the most pronounced inhibitory effect was on PxGSTσ. Metabolism assays displayed that PxGSTσ was superior to other test PxGSTs in metabolizing TFP. The molecular docking of TFP and PxGSTσ revealed that the H-bond provided by the sidechains of Tyr107 and Tyr162 were key to the detoxification of TFP by PxGSTσ. Further tests using mutant PxGSTσ proteins at the sites of Tyr107 (PxGSTσY107A) and Tyr162 (PxGSTσY162A) corroborated that the individual replacement of Tyr107 and Tyr162 could greatly weaken the binding and metabolic abilities to TFP.
CONCLUSION: Metabolic interactions between the Plutella xylostella (L.) GSTs (PxGSTs) and TFP were deciphered. This study illustrates the molecular metabolism mechanism of PxGSTσ towards TFP and provides theoretical underpinnings for the design and optimization of novel TFP-like insecticides.